type strain atcc 14126  (ATCC)

Journal: Scientific Reports

Article Title: Revisiting bioluminescence and sucrose utilization in aquatic pathogens Vibrio harveyi and V. campbellii using genome-wide in silico mapping and phenotyping

doi: 10.1038/s41598-026-37651-3

Figure Lengend Snippet: Gel picture showing presence of luxA ( A and B ) and scrA ( C and D ) gene. ( A ) and ( B ): M, 100 bp ladder; L1 to L18 represents 18 V . harveyi strains (L1, ATCC 14126; L2, LMG 7890; L3, LMG 19643; L4, SB1; L5, V2301; L6, V2426; L7, V2427; L8, V2432; L9, V2437; L10, V2438; L11, V2439; L12, V2441; L13, V2452; L14, Y/12–23; L15, Y2/12–23; L16, FCD2023/4; L17, FCD2023/7; L18, FCD2025/2) L19, Negative control for V. harveyi ; L20 to L50 represents 31 V . campbellii strains (L20, LMG11216/CAIM 519 T; L21, BAA-1116; L22 to L50, 29 strains between LB1 to LB516; L51, Negative control for V. campbellii ). ( C )and ( D ): Distribution of scrA gene. M; 100 bp ladder; L1 to L18 represents 18 V . harveyi strains having desirable 698 bp amplicon. L19, Negative control for V. harveyi ; L20 to L50 represents 31 V . campbellii strains with missing scrA gene. L51: Positive control for scrA using V. alginolyticus . L52: Negative control for V. campbellii

Article Snippet: Of these lux-positive strains, only the type strain ATCC 14126 was available for phenotypic testing, and it exhibited luminescence consistent with its genomic profile (Fig. B).

Techniques: Negative Control, Amplification, Positive Control

Journal: Scientific Reports

Article Title: Revisiting bioluminescence and sucrose utilization in aquatic pathogens Vibrio harveyi and V. campbellii using genome-wide in silico mapping and phenotyping

doi: 10.1038/s41598-026-37651-3

Figure Lengend Snippet: Pangenome characteristics of Vibrio harveyi and V. campbellii (A ) Distribution of pangenome statistics (core, soft-core, shell, cloud and total genes) in 315 genomes datasets. ( B ) Distribution of pangenome statistics in the curated dataset of 82 genomes. ( C ) Pangenome accumulation curve based on 315 genomes. ( D ) Pangenome accumulation curve based on the curated dataset of 82 genomes

Article Snippet: The analysis revealed that four strains of V. harveyi (BSW5, BSW7, 1DA3 and KC13.17.5) were misidentified as their ANIm score ranged from 88.4% to 90% with reference to V. harveyi type strain ATCC 14126 T (Fig. ; Supplementary Table ).

Techniques:

Journal: Scientific Reports

Article Title: Revisiting bioluminescence and sucrose utilization in aquatic pathogens Vibrio harveyi and V. campbellii using genome-wide in silico mapping and phenotyping

doi: 10.1038/s41598-026-37651-3

Figure Lengend Snippet: Comparative analysis of genomic parameters between Vibrio harveyi and V. campbellii and among V. campbellii phylogenetic groups ( A – C ) Comparative analysis of ( A ) GC content, ( B ) genome size, and ( C ) number of protein-coding genes between V. harveyi and V. campbellii . ( D – F ) Corresponding comparisons of ( D ) GC content ( E ) genome size and ( F ) number of protein coding-genes among Gr-L, Gr-LM, and Gr-LD groups of V. campbellii . Difference in genomic parameters between V. harveyi and V. campbellii were analysed using a two-tailed independent t-test. Genomic indices among three groups of V. campbellii were compared using one-way ANOVA followed by Tukey’s multiple comparison. V. campbellii strains were classified into three phylogenetic groups; Gr-L (luminescent), Gr-LD (luminescence-defective), and Gr-LM (luminescent with mobile genetic elements) based on core genome phylogeny and synteny organization. Data represent mean ± SE. The level of significance is indicated as: P < 0.05 (*), P < 0.01 (**), and P < 0.001 (***)

Article Snippet: The analysis revealed that four strains of V. harveyi (BSW5, BSW7, 1DA3 and KC13.17.5) were misidentified as their ANIm score ranged from 88.4% to 90% with reference to V. harveyi type strain ATCC 14126 T (Fig. ; Supplementary Table ).

Techniques: Two Tailed Test, Comparison

Journal: Scientific Reports

Article Title: Revisiting bioluminescence and sucrose utilization in aquatic pathogens Vibrio harveyi and V. campbellii using genome-wide in silico mapping and phenotyping

doi: 10.1038/s41598-026-37651-3

Figure Lengend Snippet: Distribution of luminescence and sucrose operon genes in Vibrio harveyi and V. campbellii Strains sequenced in the present study has been shown in blue color. Luminescence operon ( luxCDABEGH ) has been shown as blue color shaded circle while scrRAKB operon as red shaded square. The empty circle/square represents the absence of gene. The tree was visualized and annotated using iTOL. Vh, V. harveyi ; Vc, V. campbellii

Article Snippet: The analysis revealed that four strains of V. harveyi (BSW5, BSW7, 1DA3 and KC13.17.5) were misidentified as their ANIm score ranged from 88.4% to 90% with reference to V. harveyi type strain ATCC 14126 T (Fig. ; Supplementary Table ).

Techniques:

Journal: Scientific Reports

Article Title: Revisiting bioluminescence and sucrose utilization in aquatic pathogens Vibrio harveyi and V. campbellii using genome-wide in silico mapping and phenotyping

doi: 10.1038/s41598-026-37651-3

Figure Lengend Snippet: Syntenic organization of luminescence operon ( A ) and scrRAKB operon ( B ) in Vibrio harveyi and V. campbellii Vh, V. harveyi ; Vc, V. campbellii ; Va, V. alginolyticus. T3SS indicates start of T3SS operon by VopD. The luxCDABEGH operon and scrRAKB operon is represented by orange color. Blue color is representing the presence of transposable elements. The tree was generated using EasyFig and annotated using InkScape (version 1.4.2)

Article Snippet: The analysis revealed that four strains of V. harveyi (BSW5, BSW7, 1DA3 and KC13.17.5) were misidentified as their ANIm score ranged from 88.4% to 90% with reference to V. harveyi type strain ATCC 14126 T (Fig. ; Supplementary Table ).

Techniques: Generated